Detection of extended-spectrum beta-lactamases with Etest and double-disc potentiation methods

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منابع مشابه

Comparison of double disc and combined disc method for the detection of extended spectrum beta lactamases in enterobacteriaceae.

OBJECTIVE To compare double disc approximation and combined disc method for their ability to detect extended spectrum b lactamase (ESBL) production in enterobacteriaceae and determine the percentage of isolates which are falsely reported as sensitive in absence of ESBL detection, in a clinical microbiology laboratory of a tertiary care hospital between September-October 2002. METHODS Selected...

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Extended - spectrum Beta - lactamases

Beta-lactamase enzymes (BLs) have become the most worrisome mediators of antimicrobial resistance expressed by gram-negative bacteria (GNB). The multiple families of BLs have in common the ability to hydrolyze the -lactam rings of one or more members of the penicillins, cephalosporins, cephamycins, carbapenems, and monobactams. Extended-spectrum -lactamases (ESBLs) are a subset of BLs that conf...

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Extended-spectrum beta-lactamases.

At least 30 extended-spectrum beta-lactamases (ESB) have emerged responsible for resistance to indigestible beta-lactams (C3G) since their discovery in West Germany in 1983. Most of them are produced by enterobacteria and essentially K. pneumoniae which appeared susceptible to oxyimino-beta-lactams. A double-disk test was useful to detect such nosocomial isolates of enterobacteria (urines, bloo...

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Detection of Ampc and Extended-Spectrum Beta-Lactamases in Clinical Isolates of Pseudomonas Aeruginosa from Patients with Cystic Fibrosis

ABSTRACT        Background and Objectives: Pseudomonas aeruginosa is the most frequent opportunistic pathogen isolated from the sputum of patients with cystic fibrosis (CF). Resistance to β -lactam antibiotics may arise from over expression of the naturally occurring AmpC cephalosporinases or acquired extended-spectrum β-lactamases (ESBL). The aim of...

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Extended double disc synergy testing reveals a low prevalence of extended-spectrum beta-lactamases in Enterobacter spp. in Vienna, Austria.

OBJECTIVES The aims of this study were to determine the prevalence of extended-spectrum beta-lactamases (ESBLs) in AmpC-carrying Enterobacter spp. in a tertiary care university hospital in Vienna, Austria, and to implement a cost-effective strategy to detect ESBLs in this particular genus on a routine basis. METHODS Clinical Enterobacter isolates (n=208) were investigated by means of (i) an i...

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ژورنال

عنوان ژورنال: Journal of Antimicrobial Chemotherapy

سال: 2000

ISSN: 1460-2091

DOI: 10.1093/jac/46.2.327